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@@ -0,0 +1,1016 @@
+{
+ "uuid": "XLSX-29796c5e-00be-414f-8304-b40efd9d8d5a",
+ "owner": {
+ "substance": {
+ "uuid": "XLSX-fa48e134-54cf-3417-a63a-6f744191cec2"
+ },
+ "company": {
+ "uuid": "XLSX-4bb81f08-059d-32ad-b5fd-71c6bcc6e0db",
+ "name": "FP7 MARINA"
+ }
+ },
+ "citation": {
+ "title": "http://dx.doi.org/10.1371/journal.pone.0127174",
+ "year": "2015",
+ "owner": "P32 - KI"
+ },
+ "protocol": {
+ "topcategory": "TOX",
+ "category": {
+ "code": "NPO_1709_SECTION",
+ "term": "http://purl.bioontology.org/ontology/npo#NPO_1709",
+ "title": "NPO_1709 LDH Release Assay"
+ },
+ "endpoint": "Cell viability - cell membrane damage: Lactate dehydrogenase assay (LDH) (CytoTox 96® Non-radioactive cytotoxicity assay - Promega, G1780) ",
+ "guideline": [
+ "Cytotoxicity using human monocyte-derived macrophages (HMDM)"
+ ]
+ },
+ "parameters": {
+ "CHMO:0002774": "N",
+ "CLO_0000031": "HMDM",
+ "CLO_0000031 EFO_0004443": "Karolinska University Hospital, Stockholm, Sweden - buffy coats from healthy adult blood donors ",
+ "Cell culture conditions - Serum": "FBS (Gibco, 10500) ",
+ "Cell culture conditions - Serum concentration in growth medium": 0.1,
+ "Cell culture conditions - Serum concentration in treatment medium": "0,05%",
+ "Cell line/Type - Full name": "Primary human monocyte-derived macrophages (HMDM)",
+ "Dispersion agent": "water with 0,05% BSA",
+ "NPO_1952": "Y",
+ "NPO_1961": "Y",
+ "NPO_1969 OBI_0000272": "NANOGENOTOX Dispersion protocol (http://www.nanogenotox.eu/files/PDF/web%20nanogenotox%20dispersion%20protocol.pdf) ",
+ "OBI_0001911 BAO_0000114": "RPMI (Sigma, R0833)"
+ },
+ "reliability": {
+ "r_isRobustStudy": "false",
+ "r_isUsedforClassification": "false",
+ "r_isUsedforMSDS": "false",
+ "r_purposeFlag": null,
+ "r_studyResultType": "experimental result",
+ "r_value": null
+ },
+ "interpretation": {
+ "result": null
+ },
+ "effects": [
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 0,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 1",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 100
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 1,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 1",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 96.244
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 5,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 1",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 97.04
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 10,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 1",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 92.331
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 25,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 1",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 92.2
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 50,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 1",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 94.548
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 75,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 1",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 91.351
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 100,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 1",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 88.663
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 0,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 100
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 1,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 100.657
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 5,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 102.033
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
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+ "loValue": 10,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 102.058
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
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+ "loValue": 25,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
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+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 101.331
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 50,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 103.58
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
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+ "loValue": 75,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 103.111
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 100,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 102.116
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
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+ "loValue": 0,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 100
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 1,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 106.119
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 5,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
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+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 99.162
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 10,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
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+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 101.619
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 25,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 93.146
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
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+ "loValue": 50,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 91.801
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
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+ "loValue": 75,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 98.457
+ }
+ },
+ {
+ "endpoint": "% cell viability",
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+ "Concentration": {
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+ "loValue": 100,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
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+ "loValue": 6,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 78.147
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 0,
+ "unit": "µg/ml"
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+ "Replicate": "Replicate 1",
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+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 100
+ }
+ },
+ {
+ "endpoint": "% cell viability",
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+ "unit": "µg/ml"
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+ "unit": "h"
+ }
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+ "result": {
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+ "loValue": 85.847
+ }
+ },
+ {
+ "endpoint": "% cell viability",
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+ "loValue": 5,
+ "unit": "µg/ml"
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+ "Replicate": "Replicate 1",
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+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 84.564
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 10,
+ "unit": "µg/ml"
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+ "Replicate": "Replicate 1",
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+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 68.466
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
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+ "unit": "µg/ml"
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+ "Replicate": "Replicate 1",
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+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 69.795
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
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+ "loValue": 50,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 1",
+ "Time point": {
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+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 71.551
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
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+ "loValue": 75,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 1",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 42.512
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 100,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 1",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 58.861
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 0,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 100
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 1,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
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+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 95.042
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 5,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 99.02
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 10,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 77.547
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 25,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 73.485
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 50,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 64.706
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 75,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 72.679
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 100,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 2",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 56.168
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 0,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 100
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 1,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 109.581
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 5,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 116.075
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 10,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 95.767
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 25,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 90.067
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 50,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 100.967
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 75,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 96.509
+ }
+ },
+ {
+ "endpoint": "% cell viability",
+ "conditions": {
+ "Concentration": {
+ "loQualifier": "=",
+ "loValue": 100,
+ "unit": "µg/ml"
+ },
+ "Replicate": "Replicate 3",
+ "Time point": {
+ "loQualifier": "=",
+ "loValue": 24,
+ "unit": "h"
+ }
+ },
+ "result": {
+ "unit": "%",
+ "loValue": 96.421
+ }
+ }
+ ]
+}
generated by cgit v1.2.3 (git 2.25.1) at 2024-05-04 14:15:23 +0000