{
  "uuid": "XLSX-012fa9ed-275d-43bf-996d-20ee50308991",
  "owner": {
    "substance": {
      "uuid": "XLSX-aacdfad8-f3d3-39f2-b662-0d8084e283ef"
    },
    "company": {
      "uuid": "XLSX-4bb81f08-059d-32ad-b5fd-71c6bcc6e0db",
      "name": "FP7 MARINA"
    }
  },
  "citation": {
    "title": "http://dx.doi.org/10.1371/journal.pone.0127174",
    "year": "2015",
    "owner": "P32 - KI"
  },
  "protocol": {
    "topcategory": "TOX",
    "category": {
      "code": "NPO_1709_SECTION",
      "term": "http://purl.bioontology.org/ontology/npo#NPO_1709",
      "title": "NPO_1709 LDH Release Assay"
    },
    "endpoint": "Cell viability - cell membrane damage: Lactate dehydrogenase assay (LDH)  (CytoTox 96® Non-radioactive cytotoxicity assay - Promega, G1780) ",
    "guideline": [
      "Cytotoxicity using human monocyte-derived macrophages (HMDM)"
    ]
  },
  "parameters": {
    "CHMO:0002774": "N",
    "CLO_0000031": "HMDM",
    "CLO_0000031 EFO_0004443": "Karolinska University Hospital, Stockholm, Sweden - buffy coats from healthy adult blood donors ",
    "Cell culture conditions - Serum": "FBS (Gibco, 10500) ",
    "Cell culture conditions - Serum concentration in growth medium": 0.1,
    "Cell culture conditions - Serum concentration in treatment medium": "0,05%",
    "Cell line/Type - Full name": "Primary human monocyte-derived macrophages (HMDM)",
    "Dispersion agent": "water with 0,05% BSA",
    "NPO_1952": "Y",
    "NPO_1961": "Y",
    "NPO_1969 OBI_0000272": "NANOGENOTOX Dispersion protocol (http://www.nanogenotox.eu/files/PDF/web%20nanogenotox%20dispersion%20protocol.pdf) ",
    "OBI_0001911 BAO_0000114": "RPMI (Sigma, R0833)"
  },
  "reliability": {
    "r_isRobustStudy": "false",
    "r_isUsedforClassification": "false",
    "r_isUsedforMSDS": "false",
    "r_purposeFlag": null,
    "r_studyResultType": "experimental result",
    "r_value": null
  },
  "interpretation": {
    "result": null
  },
  "effects": [
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 100
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 97.542
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 96.65
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 10,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 94.321
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 82.461
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 68.18
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 58.725
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 39.835
      }
    },
    {
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          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
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          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 100
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 98.295
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
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        "unit": "%",
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    },
    {
      "endpoint": "% cell viability",
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          "unit": "µg/ml"
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          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 93.735
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    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 83.795
      }
    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 64.729
      }
    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
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          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
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          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 53.187
      }
    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 43.117
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
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          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 100
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 99.92
      }
    },
    {
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          "unit": "µg/ml"
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          "unit": "h"
        }
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    },
    {
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          "unit": "µg/ml"
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          "unit": "h"
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        "unit": "%",
        "loValue": 82.094
      }
    },
    {
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          "loValue": 25,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 56.049
      }
    },
    {
      "endpoint": "% cell viability",
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          "unit": "µg/ml"
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          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 25.874
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    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
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        "unit": "%",
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    },
    {
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          "loValue": 100,
          "unit": "µg/ml"
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          "unit": "h"
        }
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        "unit": "%",
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      }
    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
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          "unit": "µg/ml"
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          "unit": "h"
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        "unit": "%",
        "loValue": 100
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    },
    {
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          "unit": "h"
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        "unit": "%",
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    {
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          "loValue": 10,
          "unit": "µg/ml"
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          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 79.01
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    },
    {
      "endpoint": "% cell viability",
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          "unit": "µg/ml"
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          "unit": "h"
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        "unit": "%",
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    },
    {
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          "unit": "µg/ml"
        },
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          "unit": "h"
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        "unit": "%",
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          "unit": "µg/ml"
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          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 44.124
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    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
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          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
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          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 28.98
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    },
    {
      "endpoint": "% cell viability",
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          "loValue": 0,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 100
      }
    },
    {
      "endpoint": "% cell viability",
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          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
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          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 91.224
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    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 77.783
      }
    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
          "loValue": 10,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 75.223
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
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          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 56.581
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    },
    {
      "endpoint": "% cell viability",
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          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 35.798
      }
    },
    {
      "endpoint": "% cell viability",
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          "unit": "µg/ml"
        },
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          "loQualifier": "=",
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          "unit": "h"
        }
      },
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        "unit": "%",
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    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 25.894
      }
    },
    {
      "endpoint": "% cell viability",
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          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
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          "unit": "h"
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        "unit": "%",
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    {
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          "unit": "µg/ml"
        },
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        "Time point": {
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          "unit": "h"
        }
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    },
    {
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          "unit": "µg/ml"
        },
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          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 121.997
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    },
    {
      "endpoint": "% cell viability",
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          "unit": "µg/ml"
        },
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          "unit": "h"
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      },
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    },
    {
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          "unit": "µg/ml"
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        "Time point": {
          "loQualifier": "=",
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          "unit": "h"
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      },
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        "unit": "%",
        "loValue": 97.73
      }
    },
    {
      "endpoint": "% cell viability",
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          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
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          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 92.256
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    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 86.975
      }
    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
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          "unit": "h"
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        "loValue": 54.098
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    }
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}