{
  "uuid": "XLSX-502d510d-75f3-4178-ad7b-66d35eca34fc",
  "owner": {
    "substance": {
      "uuid": "XLSX-fa48e134-54cf-3417-a63a-6f744191cec2"
    },
    "company": {
      "uuid": "XLSX-4bb81f08-059d-32ad-b5fd-71c6bcc6e0db",
      "name": "FP7 MARINA"
    }
  },
  "citation": {
    "title": "http://dx.doi.org/10.1371/journal.pone.0127174",
    "year": "2015",
    "owner": "P32 - KI"
  },
  "protocol": {
    "topcategory": "TOX",
    "category": {
      "code": "BAO_0002993_SECTION",
      "term": "http://www.bioassayontology.org/bao#BAO_0002993",
      "title": "BAO_0002993 Cytotoxicity Assay"
    },
    "endpoint": "",
    "guideline": [
      "ELISA / TNF-α release in culture medium"
    ]
  },
  "parameters": {
    "CHMO:0002774": "Y",
    "CLO_0000031": "HMDM (primary cells)",
    "CLO_0000031 EFO_0004443": "ATCC",
    "Cell culture conditions - Serum": "FBS (Gibco/413105K) ",
    "Cell culture conditions - Serum concentration in growth medium": 0.1,
    "Cell culture conditions - Serum concentration in treatment medium": 0.1,
    "Cell line/Type - Full name": "human monocyte-derived macrophages (HMDM)",
    "Dispersion agent": "water with 0,05% BSA",
    "NPO_1952": "N",
    "NPO_1961": "N",
    "NPO_1969 OBI_0000272": "NANOGENOTOX Dispersion protocol (http://www.nanogenotox.eu/files/PDF/web%20nanogenotox%20dispersion%20protocol.pdf) ",
    "OBI_0001911 BAO_0000114": "RPMI (Sigma, RNBB2729 "
  },
  "reliability": {
    "r_isRobustStudy": "false",
    "r_isUsedforClassification": "false",
    "r_isUsedforMSDS": "false",
    "r_purposeFlag": null,
    "r_studyResultType": "experimental result",
    "r_value": null
  },
  "interpretation": {
    "result": null
  },
  "effects": [
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 19.209
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 49.533
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 53.391
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 10,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 50.428
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 28.303
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 16.896
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 7.728
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 6.272
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 17.204
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 33.116
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 41.917
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 10,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 50.258
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 40.343
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 20.83
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 9.504
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 5.553
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 4.515
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 8.867
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 13.772
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 10,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 29.028
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 15.238
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 5.522
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 4.2
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 4.228
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 4",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 16.168
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 4",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 26.755
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 4",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 30.424
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 10,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 4",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 61.664
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 4",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 62.196
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 4",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 14.767
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 4",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 7.709
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 4",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 7.886
      }
    }
  ]
}