{
  "uuid": "XLSX-53347e92-6eda-4e2b-be15-4210fb3bec01",
  "owner": {
    "substance": {
      "uuid": "XLSX-7011cea0-1011-3f8b-9e8a-b3289fed836a"
    },
    "company": {
      "uuid": "XLSX-4bb81f08-059d-32ad-b5fd-71c6bcc6e0db",
      "name": "FP7 MARINA"
    }
  },
  "citation": {
    "title": "http://dx.doi.org/10.1371/journal.pone.0127174",
    "year": "2015",
    "owner": "P32 - KI"
  },
  "protocol": {
    "topcategory": "TOX",
    "category": {
      "code": "BAO_0002993_SECTION",
      "term": "http://purl.obolibrary.org/obo/BAO_0002993",
      "title": "BAO_0002993 Cytotoxicity Assay"
    },
    "endpoint": "",
    "guideline": [
      "ELISA / TNF-α release in culture medium"
    ]
  },
  "parameters": {
    "CHMO:0002774": "Y",
    "CLO_0000031": "HMDM (primary cells)",
    "CLO_0000031 EFO_0004443": "ATCC",
    "Cell culture conditions - Serum": "FBS (Gibco/413105K) ",
    "Cell culture conditions - Serum concentration in growth medium": 0.1,
    "Cell culture conditions - Serum concentration in treatment medium": 0.1,
    "Cell line/Type - Full name": "human monocyte-derived macrophages (HMDM)",
    "Dispersion agent": "water with 0,05% BSA",
    "NPO_1952": "N",
    "NPO_1961": "N",
    "NPO_1969 OBI_0000272": "NANOGENOTOX Dispersion protocol (http://www.nanogenotox.eu/files/PDF/web%20nanogenotox%20dispersion%20protocol.pdf) ",
    "OBI_0001911 BAO_0000114": "RPMI (Sigma, RNBB2729 "
  },
  "reliability": {
    "r_isRobustStudy": "false",
    "r_isUsedforClassification": "false",
    "r_isUsedforMSDS": "false",
    "r_purposeFlag": null,
    "r_studyResultType": "experimental result",
    "r_value": null
  },
  "interpretation": {
    "result": null
  },
  "effects": [
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 1.893
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 19.698
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 18.521
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 10,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 18.028
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 18.415
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 19.297
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 20.887
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 22.696
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": -0.343
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 5.989
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 5.77
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 10,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 5.301
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 5.16
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 5.229
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 6.156
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 7.281
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 2.189
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 2.474
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
        "Concentration": {
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          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
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          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 2.002
      }
    },
    {
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      "conditions": {
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          "loValue": 10,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 1.786
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
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          "loValue": 25,
          "unit": "µg/ml"
        },
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          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
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        "unit": "ng/ml",
        "loValue": 1.57
      }
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          "loValue": 50,
          "unit": "µg/ml"
        },
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          "unit": "h"
        }
      },
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        "unit": "ng/ml",
        "loValue": 1.735
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
      "conditions": {
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          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
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          "loValue": 24,
          "unit": "h"
        }
      },
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        "unit": "ng/ml",
        "loValue": 1.29
      }
    },
    {
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      "conditions": {
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          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
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          "unit": "h"
        }
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          "unit": "µg/ml"
        },
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          "unit": "h"
        }
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        "unit": "ng/ml",
        "loValue": 9.502
      }
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          "unit": "µg/ml"
        },
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        }
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    },
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          "unit": "µg/ml"
        },
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          "unit": "h"
        }
      },
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        "unit": "ng/ml",
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      }
    },
    {
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          "loValue": 10,
          "unit": "µg/ml"
        },
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          "unit": "h"
        }
      },
      "result": {
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        "loValue": 8.306
      }
    },
    {
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          "unit": "µg/ml"
        },
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          "unit": "h"
        }
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    },
    {
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          "unit": "µg/ml"
        },
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          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 9.507
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
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          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 4",
        "Time point": {
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          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 14.416
      }
    },
    {
      "endpoint": "TNF-α (ng/ml)",
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        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 4",
        "Time point": {
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          "unit": "h"
        }
      },
      "result": {
        "unit": "ng/ml",
        "loValue": 15.438
      }
    }
  ]
}