{
  "uuid": "XLSX-5ce7f13f-0535-4b46-bf94-28c7566e42e6",
  "owner": {
    "substance": {
      "uuid": "XLSX-93b2da85-fc0c-315f-8d3b-77f1a7776fb4"
    },
    "company": {
      "uuid": "XLSX-4bb81f08-059d-32ad-b5fd-71c6bcc6e0db",
      "name": "FP7 MARINA"
    }
  },
  "citation": {
    "title": "http://dx.doi.org/10.1371/journal.pone.0127174",
    "year": "2015",
    "owner": "P32 - KI"
  },
  "protocol": {
    "topcategory": "TOX",
    "category": {
      "code": "NPO_1709_SECTION",
      "term": "http://purl.bioontology.org/ontology/npo#NPO_1709",
      "title": "NPO_1709 LDH Release Assay"
    },
    "endpoint": "Cell viability - cell membrane damage: Lactate dehydrogenase assay (LDH)  (CytoTox 96® Non-radioactive cytotoxicity assay - Promega, G1780) ",
    "guideline": [
      "Cytotoxicity using human monocyte-derived macrophages (HMDM)"
    ]
  },
  "parameters": {
    "CHMO:0002774": "N",
    "CLO_0000031": "HMDM",
    "CLO_0000031 EFO_0004443": "Karolinska University Hospital, Stockholm, Sweden - buffy coats from healthy adult blood donors ",
    "Cell culture conditions - Serum": "FBS (Gibco, 10500) ",
    "Cell culture conditions - Serum concentration in growth medium": 0.1,
    "Cell culture conditions - Serum concentration in treatment medium": "0,05%",
    "Cell line/Type - Full name": "Primary human monocyte-derived macrophages (HMDM)",
    "Dispersion agent": "water with 0,05% BSA",
    "NPO_1952": "Y",
    "NPO_1961": "Y",
    "NPO_1969 OBI_0000272": "NANOGENOTOX Dispersion protocol (http://www.nanogenotox.eu/files/PDF/web%20nanogenotox%20dispersion%20protocol.pdf) ",
    "OBI_0001911 BAO_0000114": "RPMI (Sigma, R0833)"
  },
  "reliability": {
    "r_isRobustStudy": "false",
    "r_isUsedforClassification": "false",
    "r_isUsedforMSDS": "false",
    "r_purposeFlag": null,
    "r_studyResultType": "experimental result",
    "r_value": null
  },
  "interpretation": {
    "result": null
  },
  "effects": [
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 100
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 99.413
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 99.916
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 10,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 99.495
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 98.373
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 97.147
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 93.705
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 82.427
      }
    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
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          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
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          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 100
      }
    },
    {
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      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 99.36
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 99.216
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    },
    {
      "endpoint": "% cell viability",
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          "unit": "µg/ml"
        },
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          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 96.477
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    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 96.174
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 94.889
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
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          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
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          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 93.054
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 77.011
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
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          "loValue": 0,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 100
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
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      },
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        "unit": "%",
        "loValue": 93.623
      }
    },
    {
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          "unit": "µg/ml"
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          "unit": "h"
        }
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    },
    {
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        "Concentration": {
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          "unit": "µg/ml"
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          "unit": "h"
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        "unit": "%",
        "loValue": 96.911
      }
    },
    {
      "endpoint": "% cell viability",
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          "loValue": 25,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 75.319
      }
    },
    {
      "endpoint": "% cell viability",
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          "unit": "µg/ml"
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          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 66.08
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    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 6,
          "unit": "h"
        }
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        "unit": "%",
        "loValue": 41.821
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    },
    {
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          "loValue": 100,
          "unit": "µg/ml"
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          "unit": "h"
        }
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        "unit": "%",
        "loValue": 39.382
      }
    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
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          "loValue": 0,
          "unit": "µg/ml"
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          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 100
      }
    },
    {
      "endpoint": "% cell viability",
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          "unit": "µg/ml"
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          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 89.377
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    },
    {
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          "loValue": 10,
          "unit": "µg/ml"
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          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 89.038
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    },
    {
      "endpoint": "% cell viability",
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          "unit": "µg/ml"
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          "unit": "h"
        }
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        "unit": "%",
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    },
    {
      "endpoint": "% cell viability",
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          "loValue": 50,
          "unit": "µg/ml"
        },
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          "unit": "h"
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        "unit": "%",
        "loValue": 70.673
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    },
    {
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          "unit": "µg/ml"
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        "Time point": {
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          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 55.286
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    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 1",
        "Time point": {
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          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 36.018
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    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 100
      }
    },
    {
      "endpoint": "% cell viability",
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          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
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        "Time point": {
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          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 92.006
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    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 5,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 86.708
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 10,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 87.863
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 25,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
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          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 90.631
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    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 81.019
      }
    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 70.953
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    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
          "loValue": 100,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 2",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 54.229
      }
    },
    {
      "endpoint": "% cell viability",
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          "loQualifier": "=",
          "loValue": 0,
          "unit": "µg/ml"
        },
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          "loValue": 24,
          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 100
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    },
    {
      "endpoint": "% cell viability",
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          "loQualifier": "=",
          "loValue": 1,
          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
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          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 116.133
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    },
    {
      "endpoint": "% cell viability",
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          "loValue": 5,
          "unit": "µg/ml"
        },
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          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
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        "unit": "%",
        "loValue": 127.122
      }
    },
    {
      "endpoint": "% cell viability",
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          "loQualifier": "=",
          "loValue": 10,
          "unit": "µg/ml"
        },
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          "unit": "h"
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      },
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        "unit": "%",
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    },
    {
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          "unit": "µg/ml"
        },
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        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 97.885
      }
    },
    {
      "endpoint": "% cell viability",
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          "loQualifier": "=",
          "loValue": 50,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 93.242
      }
    },
    {
      "endpoint": "% cell viability",
      "conditions": {
        "Concentration": {
          "loQualifier": "=",
          "loValue": 75,
          "unit": "µg/ml"
        },
        "Replicate": "Replicate 3",
        "Time point": {
          "loQualifier": "=",
          "loValue": 24,
          "unit": "h"
        }
      },
      "result": {
        "unit": "%",
        "loValue": 81.871
      }
    },
    {
      "endpoint": "% cell viability",
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        "Concentration": {
          "loQualifier": "=",
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          "unit": "h"
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        "loValue": 57.012
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    }
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}